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The University of Tennessee Institute of Agriculture ANNUAL REPORTING FORM | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Douglas Hayes, Associate Professor Biosystems Engineering & Soil Science Annual Performance Period: January 1, 2006 to December 31, 2006 A. SUMMARY AND IMPACTS. 1. Summary. Provide an overall summary (500 words or less) of your accomplishments during the reporting period. PhD student Javier Gomez del Rio (Chem Engr) has developed 3-phase microemulsion systems containing a pH-degradable surfactant that can effectively recover and purify proteins from aqueous solutions. He has developed a thermodynamic model to describe the partitioning of the surfactants between phases as a function of temperature and surfactant concentration (manuscript in preparation). Current research is focusing upon the recovery of microemulsion-encapsulated proteins. Mayson Alkhatib completed her PhD at University of Alabama in Huntsville in 2006; Gomez will complete his PhD in 2007. Manuscripts (3) have been written by Alkhatib from her thesis work. Small-Angle Neutron Scattering (SANS) results, obtained at NIST, Gaithersburg, MD in 2 sessions in 2006 (obtained through acceptance of peer-reviewed proposals), in collaboration with Drs. Volker Urban and J.S. Lin of ORNL, are in the final stages of analysis, and will be included in the 3 manuscripts. Manuscripts (2) were submitted from previous work related to this project, with one accepted for publication and the second under review (revised manuscript, minor revisions required). Proposals will be prepared in 2007. Proposals have been written in collaboration with Dr. F. Harte of Food Science for the use of surfactants in the formation of casein micelles. Regarding the lipase-catalyzed environmentally-friendly synthesis of saccharide-fatty acid esters as value-added bio-based products, graduate student Oriana Obiri (BESS) completed her Masters Thesis. She successfully designed and operated a bioreactor prototype (manuscript submitted). Two proposals (NSF and USDA) were written, with the latter being funded. Dr. Sang-Hyun Pyo will begin work as a postdoctoral research associate beginning in January, 2007. Research on lipase-catalyzed synthesis of polyesters of hydroxy fatty acids has continued, focusing on copolymerization reactions. A proposal on this topic submitted to NIH received a very good “priority score” of 148 from a research panel, and thus has a good chance of being funded. Research being conducted by Ms. Lindsey Kline (MS student, BESS) in collaboration with Dr. Nicole Labbe (Tennessee Forest Product Center), has demonstrated that the ability to solubilize lignocellulosic biomass in ionic liquids may be useful in developing a colorimetric assay for the rapid measurement of lignin content for biomass. In addition, Ms. Kline has demonstrated that cellulose can be enzymatically hydrolyzed in ionic liquids, which may be of great interest for developing a safer, more accurate method for determining the cellulose and hemicellulose content of biomass, and as a pretreatment of biomass in the production of bioethanol (proposal in preparation). In collaboration with Dr. Philip Ye and his laboratory, we have determined differences in the chemical composition of different anatomical components of corn stover (node, internode, leaves, etc). Regarding malaria bed nets, a manuscript for publication has been written and will be submitted soon. A possible collaboration between colleagues at Texas Tech, Dr. Larry Wadsworth, and Hayes on this topic have been discussed. We have collaborated with Dr. David Compton (USDA/ARS, Peoria, IL) on the use of urea inclusion compounds to purify a bio-sunscreen product. 2. Impacts. List up to three significant impacts of your program. Impact statements should be one to three sentences and should be written in a way that would be meaningful to the public. Purification of proteins from fermentation broths at >95% yield has occurred when employing a novel method based on extraction using 3-phase microemulsion systems and pH-degradable surfactants. This technique can potentially be more cost-effective, easily scaled up, and rapid compared to competing technologies. A bench-scale prototype bioreactor system has been developed and tested that is capable of synthesizing technical-grade biocompatible emulsifiers for food and cosmetic products has been synthesized from agricultural feedstocks, fatty acids and monosaccharides, via biocatalysis in an environmentally-friendly, solvent-free, procedure. A mathematical model was developed which effectively described the chemical reaction data. Poorly-soluble biopolymers derived from biomass, such as cellulose, lignin, and whole corn stover, have been effectively solubilized in ionic liquids. This capability will allow for more simple, rapid and safe methods for analyzing the composition of lignocellulosic biomass. B. PROJECTS AND PROGRAMS (EXTENSION, RESEARCH AND TEACHING). As applicable, list title, names of participants (PIs, Leaders, Co-PIs, Co-leaders, Collaborators etc.), project number, sponsor, your role ( leader/co-leader/collaborator) and your percent effort on the project as stated in the project description or document. 1. pH-Degradable Surfactants for Tunable Water-in-Oil Microemulsions, D. G. Hayes (PI/leader), Volker S. Urban (ORNL, collaborator), J.S. Lin (ONRL, collaborator), NSF, 10% effort 2. Synthesis of Biosurfactant Fatty Acid Esters and Biolubricant Star Polymers via Biocatalysis, D. G. Hayes (PI / leader), USDA (+NIH proposal pending), 10% effort 3. Wash-Durable Malaria Bed Nets, D. G. Hayes (PI / leader), K. Chittur (UAH, co-PI), D. Mount (CDC, collaborator), S. Smith (CDC, collaborator), L. C. Wadsworth (collaborator), CDC/ASPH (grant expired in 2004), 5% effort 4. Upstream Conversion of Biomass (Hayes effort: Use of ionic liquids to improve wet chemical analysis of biomass), A. Womac (PI), P. Ye (collaborator), D. G. Hayes (collaborator), N. Labbe (FPC, collaborator), and other collaborators, USDA/DOE, 5% effort 5. Fractionation of Lipids and Polymers via Urea Inclusion Compounds, D. G. Hayes (PI), J. M. Van Alstine (Amersham Biosciences, Uppsala, Sweden; collaborator), David Compton (USDA/ARS/NCAUR, Peoria, IL, collaborator), no funding, 2% effort. C. INSTRUCTION, TEACHING AND EXTENSION EDUCATION. Detail credit and non-credit instruction and teaching activities. 1. College credit instruction. List courses in which you were an instructor or guest lecturer during the current calendar year. Include special topics, independent study and internship courses. Provide course number and title, your involvement, semester(s), credit hours and number of students. Please append a summary of teaching evaluations (student and/or peer) if this information is available.
2. Formal continuing education (certification programs, seminar series, in-service training, field days, faculty and/or staff training, journal club, etc.). Provide descriptive title of activity, your involvement, number of participants or students involved, number and duration of each session.
3. Extension presentations and workshops. Indicate title, type, and scope of presentation; number of times presented; attendance estimates. Include evidence of effectiveness (e.g. evaluation summaries) where applicable.
4. Public addresses to lay audiences (TV/radio presentations, service groups, etc.). Indicate title, type, scope of presentation; number of times presented; attendance estimates. Include evidence of effectiveness (e.g. evaluation summaries) where applicable.
5. Presentations at professional meetings, without published abstracts. List presenter, co-authors (if any), title, date and name of meeting (list scientific oral or poster presentations having published abstracts or proceedings at D5). • A.R. Womac (presenter), X.P. Ye, D.G. Hayes, C. Igathinathane, S. Klasek, P.Miu, T. Yang, M. Yu, S. Sokhansanj, S. Narayan, "Advances in Biomass Integrated Size Reduction and Separation," 2006 Agricultural Equipment Technology Conference (Feb 12-14) , Louisville, KY • Douglas G. Hayes [speaker], "Polyol-Fatty Acid Esters: Value-Added Products Formed Using Biocatalysis and Environmentally-Friendly Processing,” American Oil Chemists’ Society Annual Meeting, St. Louis, 05/06 [Invited] • Javier Gomez del Rio and Douglas G. Hayes [speaker], " Use of Microemulsions Based on pH-Degradable Surfactants for Protein Solubilization, Release, and Purification,” American Oil Chemists’ Society Annual Meeting, St. Louis, 05/06 [Invited] • Oriana Obiri [poster presenter] and Douglas G. Hayes, "Quantification and Analysis of Lipase-Catalyzed Synthesis of Various Saccharide Oleic Acid Monoesters." American Oil Chemists’ Society Annual Meeting, St. Louis, 05/06 • X Philip Ye [Speaker], Alvin R. Womac, Doug Hayes, Lu Liu, Lindsey Kline, Shahab Sokhansanj and S. Narayan “Fast biomass compositional analysis using Fourier Transform Near-infrared Technique,” 2006 ASABE ANNUAL INTERNATIONAL MEETING – July 9-12, 2006 – Portland, Oregon • Javier A. Gomez del Rio [poster presenter] and Douglas G. Hayes, “Partition of a Cleavable, 1,3-Dioxolane, Nonionic Surfactants in 2- and 3-Phase Water-Isooctane Microemulsion Systems,” 80th ACS Colloid and Surface Science Symposium, Boulder, CO, 05/06 • Javier A. Gomez del Rio [poster presenter] and Douglas G. Hayes, “Protein extraction by WIII system formed from binary Aerosol-OT / Cleavable, 1,3-Dioxolane, Nonionic Surfactants,” 80th ACS Colloid and Surface Science Symposium, Boulder, CO, 05/06 • Mayson H. Alkhatib [poster presenter] and Douglas G. Hayes, “Phase behavior of mixed Ck2-13/C8âG1,” 80th ACS Colloid and Surface Science Symposium, Boulder, CO, 05/06 • Mayson H. Alkhatib [speaker], and Douglas G. Hayes, “Physicochemical characterization of mixed pH-degradable Ck2-13/AOT surfactants microemulsions: phase diagrams, electrical conductivity and FTIR spectroscopy,” 80th ACS Colloid and Surface Science Symposium, Boulder, CO, 05/06 • Fei Zhuang, Krishnan K. Chittur, Dwight L. Mount, Stephen C. Smith, Larry C. Wadsworth, and Douglas G. Hayes [speaker], “Wash-Durable Malaria Bed Nets via In Situ Polymerization,” AATCC Conference, Atlanta, 11/06 [Invited] 6. Student mentoring. Fill in the boxes to indicate number of students in each category.
7. Student or commodity club activities (e.g. club advisor, team competitions, honor or professional societies, etc.). List the club or group; your role with the club or group; and the activities, honors or awards received by this group under your mentorship. 8. Other instructional and teaching activities. Co-Advised design project (along with R. M. Counce and P. D. Frymier) for Chem Engr 490, "Process Synthesis and Analysis for Production of Recombinant Human Insulin-Like Growth Factor I: A Case Study," student leader = Nathan Jansen D. PUBLICATIONS. List only publications that were published during the annual reporting period (for #8, submitted during reporting period). Format citations as typical and appropriate for outlet. If a co-author(s) is a student or was working under your direct supervision, place an asterisk after that person's name. 1. Refereed articles or refereed reviews appearing in peer-reviewed professional journals. Hayes, D. G. 2006. Effect of Temperature Programming on the Performance of Urea Inclusion Compound-Based Free Fatty Acid Fractionation. Journal of the American Oil Chemists' Society, 83:253-259. Kelly, A. R.*, and D. G. Hayes. 2006. Lipase-Catalyzed Synthesis of Polyhydric Alcohol-Poly(Ricinoleic Acid) Ester Star Polymers. Journal of Applied Polymer Science, 101:1646-1656. 2. Book chapters or books. Hayes, D. G. 2006. Biocatalytic Synthesis of Ricinoleic Acid Star Polymers: “Green” Manufacturing of Potentially Valuable Lubricant Additives and Drug Delivery Materials. in Degradable Polymers and Materials (ACS Symposium Series 939), edited by K. Khemani and C. Scholz, Washington, DC, ACS, pp. 126-139 (peer reviewed). Hayes, D. G. 2006. Lipid Separations Using Packed-Column Supercritical Fluid Chromatography. in New Techniques and Applications in Lipid Analysis and Lipidomics, edited by M. Mossaba, J. Kramer, J.T. Brenna, and R. McDonald, Champaign, IL, AOCS Press, pp. 239-259 (Chapt. 10). 3. Bulletins, reports, circulars, pamphlets, factsheets. 4. Popular press, trade, UTIA magazine or newsletter articles. 5. Abstracts from scientific or discipline meetings, papers from conference proceedings, etc. Hayes, D. G., and L. C. Wadsworth. 2006. "Wash-Durable Malaria Bednets through In-Situ Curing of Latex Polymer onto Nets for Entrapment of Pyrethroid, AATCC IC&E 2006, Atlanta, GA, October 31-November 2, 2006. Klasek, S. E., A. R. Womac, D. C. Yoder, and D. G. Hayes. 2006. Terminal velocity determination for component separation of biomass.. Paper No. 066218, American Society of Agricultural and Biological Engineers, St. Joseph, MI. Liu, L., X. P. Ye, L. M. Kline, D. G. Hayes, A. R. Womac, and S. Sokhansanj. 2006. Fast biomass compositional analysis using fourier transform near-infrared technique. Paper No. 066155, American Society of Agricultural and Biological Engineers, St. Joseph, MI. 6. Theses/Dissertations completed by students that you directed. Klasek, S. E., and *. 2006. Terminal Velocity Determinations for Component Separation of Biomass. A Thesis Presented for the Master of Science Degree, The University of Tennessee, Knoxville . Obiri, O. O.* 2006. SYNTHESIS OF LIPASE-CATALYZED SACCHARIDE-FATTY ACID ESTERS USING A PACKED BED BIOREACTOR SYSTEM WITH CONTINUOUS RE-CIRCULATION OF REACTION MEDIUM: A CONTINUATION OF BATCH-MODE-RELATED RESEARCH. A Thesis Presented for the Master of Science Degree The University of Tennessee, Knoxville. Alkhatib, M. H.* 2006. PH-DEGRADABLE 1, 3-DIOXOLANE SURFACTANTS: SYNTHESIS AND APPLICATION IN MIXED SURFACTANT SYSTEMS. PhD Thesis, Biotechnology PhD Program, University of Alabama in Huntsville. 7. Other publications. 8. Articles submitted during the reporting period that are in review, in press or rejected. Please do not list articles in preparation. E. FUNDING. Format each entry as a citation, include as applicable: investigators (with percent effort shown on grant or contract document), title, source/agency. 1. Grant and contract proposals submitted but not awarded during this reporting period. Indicate date of submission and status (still pending, rejected). Hayes, D. G. (35%), R.R. Gerhardt (35%), L. C. Wadsworth (30%). Effective, Inexpensive, and Environmentally-Friendly Apparel for Mosquito Protection. SARE. 6/1/06. $208,762. (rejected) Hayes, D. G. (100%). Bioreactor Designs for . National Science Foundation. 1/18/06. $364,656. (rejected) Federico M. Harte (50%), D. G. Hayes (25%), David C. Joy (25%). NER: Physiochemical Stimuli determining the Nanostructure of the Casein Micelle from Bovine Milk.. NSF. 11/15/06. $129,601. (rejected) Federico M. Harte (50%), D. G. Hayes (25%), David C. Joy (15%), Michael Sepaniak (10%). CBET - Nanoscale: Exploratory Research. NSF. 11/15/06. $129,601. (rejected) 2. Grants and contracts awarded during this reporting period. List inclusive period of support (start and end dates) and total amount of award. Hayes, D. G. (50%), Volker S. Urban (30%), J. S. Lin (10%), Mayson Alkhatib (5%), J. A. Gomez del Rio (5%). SANS Analysis to Determine Key Parameters for Characterization of pH-Degradable Microemulsion System. NIST. 6/6/06-6/8/06. $2,000. Hayes, D. G. (100%). Comparison of Water Removal Strategies Contained in Bioreactor Sys. Designs for the Green Synthesis . USDA CSREES. 8/15/06-8/14/09. $308,740. Hayes, D. G. (50%), Volker S. Urban (30%), J.S. Lin (5%), J. A. Gomez del Rio (15%). SANS Analysis to Characterize pH-Degradable Microemulsion Systems: Effect of Ionic Strength, pH, and. NIST. 9/11/06-9/13/06. $1. 3. Grants and contracts awarded in past years that continued into this reporting period. List inclusive period of support (start and end dates) and total amount of award. Hayes, D. G. (100%). Rapid and Selective Protein Purification via pH-Tunable Water-in-Oil Microemulsions. National Science Foundation. 1/1/03-8/31/07. $331,000. Womac, A. R. (49%), D. G. Hayes (26%), X. P. Ye (26%). Integrated Size Reduction and Separation to Pre-Fractionate Biomass. US DOE. 10/1/04-9/30/07. $717,399. 4. Gifts. List recipients, donor, items or amount donated and dollar equivalent received during the reporting period. 5. Grant reports submitted. List authors, title, to whom submitted, date submitted. Hayes, D. G., Rapid and Selective Protein Purification via pH-Tunable Water-in-Oil Microemulsions, NSF, 06/06 6. Other (e.g. sales, royalties). F. OTHER CREATIVE ACTIVITIES.
G. SERVICE. List client outreach activities (both public and private sectors) and professional service.
H. PROFESSIONAL DEVELOPMENT (meetings, workshops, classes, trips, etc.). List the event or activity, sponsor, duration of event and your role. I. PERSONNEL SUPERVISION. List the postdoctoral fellows, research associates, research assistants, technicians, extension associates, extension assistants, graduate assistants, undergraduate students, clerical personnel or others that you supervised during the year.
J. HONORS AND AWARDS. List honors and awards received during reporting period, including the name of the organization presenting the award. Outstanding Paper Presentation, 97th American Oil Chemist's Society (AOCS)Annual Meeting, St. Louis, May, 2006 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||